日粮中添加丁酸梭菌对断奶仔猪生长性能、抗氧化能力和免疫功能的影响

doi:10.11843/j.issn.0366-6964.2018.08.010

Abstract

Abstract:

The experiment was conducted to investigate the effects of dietary Clostridium butyricum on growth performance, antioxidant capacity and immune function in weaned piglets. A total of 180 healthy crossbred weaned piglets (Duroc×(Yorkshire×Landrace), (28±1) days old, (8.07±0.84) kg initial body weight, half male and half female) were randomly allocated to 5 treatment groups, 6 replicates in each group, and 6 weaned piglets in each replicate. Dietary treatments contained:the antibiotic-free basal diet as the negative control group; the basal diet supplemented with 75 mg·kg^-1 aureomycin as positive control group; the concentration of efficacious viable germ in diets were 1.0×10⁸, 1.0×10⁹ and 5.0×10⁹CFU·kg^-1, respectively as Clostridium butyricum treatment groups. The trial lasted for 28 d. Body weight of weaned piglets were measured on 0, 14 and 28 d. During the trial, the diarrhea condition of weaned piglets were observed and recorded. One healthy piglet from each replicate was selected for collecting blood sample at the end of the trial. The levels of immune and antioxidant indexes were determined by commercial kits. The result showed that:1) The average daily gain, average daily feed intake and feed conversion ratio of weaned piglets had no significant differences in all treatment groups during1-14 d, 15-28 d and 1-28 d(P>0.05). 2) With the level of Clostridium butyricum supplementation ascending, the diarrhea rate of weaned piglets significantly linearly increased (P<0.05) during 1-14 d and 1-28 d. The feces score had significant differences among different treatment groups(P<0.05) during 15-28 d and 1-28 d. 3) Compared with negative control group, basal diet supplemented with 5.0×10⁹ CFU·kg^-1 Clostridium butyricum significantly increased the concentration of GSH-Px and T-AOC(P<0.05), basal diet supplemented with 1×10⁹ and 5.0×10⁹ CFU·kg^-1Clostridium butyricum significantly increased the concentration of T-SOD, which linearly significantly increased with the augment of Clostridium butyricum levels(P<0.05), however, there was no significant difference in MDA content among treatment groups(P>0.05). 4) The level of IgG showed a increase tendency with the level of Clostridium butyricum supplementation ascending (P<0.10). Compared with negative control group, basal diet supplemented with 5.0×10⁹CFU·kg^-1Clostridium butyricum significantly reduced the levels of TNF-α (P<0.05) and IL-6 (P<0.01) in serum of weaned piglets, while the level of IL-4 in serum significantly increased (P<0.01). Moreover, the level of IL-4 linearly significantly increased with the augment of Clostridium butyricum levels(P<0.05). There was no significant differences in IL-1β level among treatment groups(P>0.05). The results show that supplementation of 1×10⁹ -5.0×10⁹ CFU·kg^-1 Clostridium butyricum enhance antioxidant capacity and immune function of weaned piglets, which is helpful to the health level and anti-stress ability of weaned piglets.

CLC Number:

S828.5

GENG Zheng-ying, SHI Lin-xin, LIU Ping, LU Wen-qing. Effects of Dietary Supplemented with Clostridium Butyricum on Growth Performance, Antioxidant Capacity and Immune Function in Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1651-1660.

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Effects of Dietary Supplemented with Clostridium Butyricum on Growth Performance, Antioxidant Capacity and Immune Function in Weaned Piglets

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